The ladder is prepared from a plasmid containing repeats of a 1018 bp dna fragment. Supercoiled dna ladder from invitrogen,supercoiled dna ladder is suitable for sizing supercoiled dna from 2 to 16 kb by agarose gel electrophoresis 1. This visual guide allows you to easily compare the differences between dna ladders offered at goldbio in terms of size. Comparison between 1 kb plus dna ladder and 1 kb dna ladder. The ladder consists of nine bands in even increments 0. Invitrogen 10 bp dna ladder 50ug nucleic acid molecular. The singlestranded ladder can be visualized on a 6% ureapolyacrylamide gel after electrophoresis. Tridye 1 kb dna ladder is a premixed, readytoload molecular weight marker containing 3 dyes which serve as visual aids to monitor the progress of migration during agarose gel electrophoresis. Generuler 1 kb dna ladder thermo fisher scientific. Invitrogen trackit 1 kb plus dna ladder is ready to use and designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. The product is supplied in a solution of 10 mm trishcl, ph 8. Quickload 1 kb dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye.
For easy reference on agarose gels, the 100bp band is two to three times brighter than the other bands in the ladder. Thermo scientific generuler 1 kb dna ladder is recommended for sizing and approximate quantification of a doublestranded dna in the range of 250 bp to 10,000 bp on agarose gels. The 1 kb dna ladder and the 100 bp dna ladder are readytouse molecular weight markers suitable for dna fragment size determination on gel. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. The bands can be visualized by ethidium bromide staining and may also be detected on southern blots by probing with the pbr322 betalactamase ampicillinresistance gene sequence. The dna ladder consists of 14 dna fragments and is provided with 6x tritrack dna loading dye. Use the neb 1 kb ladder for size markers between 500 bp and 10 kb.
This ladder can be stained with ethidium bromide or sybr green dyes, or radiolabeled using t4 polynucleotide kinase. The ladder consists of bands that are generated from pcr and restriction enzyme digestion of double stranded dna. How to dilute dna ladders andersen lab, 2016 invitrogen 1. Invitrogen 1kb dna extension ladder 100ug nucleic acid. This dna 1 kb ladder contains 11 fragments consisting of 500 bp repeats from 0. Do not heat the trackit 1kb plus dna ladder before loading. Quickload 1 kb extend dna ladder n3239s 125 gel lanes 1. The 1 kb plus dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to 12 kb. Thermo scientific generuler 1 kb dna ladder is designed for sizing and approximate quantification of wide.
The 1kb plus dna ladder is ready to use, ranges from 25000250 base pairs, has intensities at 3k and 1k bp and is premixed with loading buffer. This ladder consists of bands containing 18 repeats of a 1018bp dna fragment. The 1 kb dna ladder, containing linear doublestranded dna fragments, is suitable for sizing doublestranded dna from 250 bp to 10,000 bp. One kb plus dna ladder is composed of 20 highly purified, doublestranded dna bands spanning 100bp to 12,000bp. Not intended for any animal or human therapeutic or diagnostic use. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed with 1ul of blueorange 6x loading dye. The ladder is composed of fourteen chromatographypurified. Trackit 1 kb plus dna ladder thermo fisher scientific. Use the 1 kb dna extension ladder to size linear doublestranded dna fragments from 500 bp40 kb. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for. By the use of these products you accept the terms and conditions of all applicable limited use label licenses.
Can be visualized on 1% to 2% agarose gels after ethidium bromide staining. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most popular markers used for dna fragment size determination. The 1 kb and 3 kb bands contain more dna to provide internal orientation. The quickload 1 kb extend dna ladder is a premixed, readytoload molecular weight dna marker containing bromophenol blue dye as a tracking dye. Contents amount storage sm0311 generuler 1 kb dna ladder 250 5 x 50 g for 500 applications, 0. The 1kb dna extension ladder consists of 8 fragments ranging in length from 1,018 to 8,144bp in 1,018bp increments, as well as bands of 506bp, 517bp, 1,636bp, 10kb, 15kb, 20kb, and 40kb. Identification of the substancemixture and of the companyundertaking product identifier 1 kb dna ladder or ruler trade namebrand invitrogen1 kb plus dna ladder synonyms deoxyribonucleic acid, aqueous solution reach number mixture, not applicable. Neb also offers a quickload version of this ladder with purple dye.
This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and. Making 1kb ladder working solution university of san diego. How can i prepare working solution with 1 kb dna ladder. Generuler 1 kb dna ladder catalog number sm0311, sm0312 pub. The 1 kb dna ladder is a unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and pcr products to yield fragments, suitable for use as molecular weight standards for electrophoresis. How to dilute dna ladders andersen lab, 2016 invitrogen 1 kb. Premixed with loading buffer ready to load room temperature stability high dna mass ladder 4. Supercoiled dna ladder from invitrogen biomedicine. Goldbios readytouse 1kb ladder serves as a clear dna standard with band fragments and greater band intensity for reference at the 3kb and 1kb bands. For optimal resolution, the recommended agarose gel concentration is 0. Band 600 bp two to three times brighter than other bands in ladder. It allows sizing and concentration estimate of dna fragments on agarose gels generated by pcr or restriction digest.
Sigmas 1kb ladder is provided in a solution of 10 mm trishcl ph 8. Comes supplied with 1 vial of gel loading dye, purple 6x, no sds. Invitrogen 1 kb plus dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 15,000 bp. This ladder consists of bands containing 1 8 repeats of a 1018bp dna fragment. Ladder consists of 15 bluntended fragments ranging in length from 100 to 1,500 bp, at 100 bp increments, and an additional fragment at 2,072 bp. This ladder has 12 evenly spaced bands ranging from 1kb to 12kb, quick orientation band at 1,650bp that forms distinct doublet with 2kb band, and seven bands of round sizes below 1kb. View more information about our dna stains technology. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. For easy reference on agarose gels, the 5kb band is two to threetimes brighter than the other bands in the ladder. The bands of the ladder each contain from 1 to 12 repeats of a 1018bp dna fragment. Use the invitrogen 1 kb plus dna ladder if you need size markers for fragments between 100 bp and 12 kb. The 1 kb dna ladder contains discrete dna fragments ranging in size from 300 bp to 10,000 bp.